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| Registros recuperados: 28 | |
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| Silva,N.P.; Christofolini,D.M.; Mortara,R.A.; Andrade,L.E.C.. |
| Cajal bodies (CB) are ubiquitous nuclear structures involved in the biogenesis of small nuclear ribonucleoproteins and show narrow association with the nucleolus. To identify possible relationships between CB and the nucleolus, the localization of coilin, a marker of CB, and of a set of nucleolar proteins was investigated in cultured PtK2 cells undergoing micronucleation. Nocodazol-induced micronucleated cells were examined by double indirect immunofluorescence with antibodies against coilin, fibrillarin, NOR-90/hUBF, RNA polymerase I, PM/Scl, and To/Th. Cells were imaged on a BioRad 1024-UV confocal system attached to a Zeiss Axiovert 100 microscope. Since PtK2 cells possess only one nucleolus organizer region, micronucleated cells presented only one or... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cajal body; Coiled body; Micronuclei; Cell nucleolus; Nucleolus organizer region; RNA polymerase I; Nucleolar proteins. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700008 |
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| Kirschbaum,Aline A.; Seriani,Robson; Pereira,Camilo D. S.; Assunção,Andrea; Abessa,Denis M. de Souza; Rotundo,Matheus M.; Ranzani-Paiva,Maria J. T.. |
| The aquatic environment receives many contaminants that can induce damages at the molecular, biochemical, cellular and physiological levels. Centropomus parallelus , an important food resource for local populations, is a predator fish that feeds on small fishes and benthic invertebrates, thus being vulnerable to the bioconcentration and biomagnification processes. This study aimed to evaluate cytogenotoxic responses in erythrocytes from C. parallelus juveniles collected in the Cananéia and São Vicente estuaries, both in winter and in summer. After anesthesia, blood samples were collected by caudal puncture. Blood smears were prepared on glass slides and stained with May-Grünwald-Giemsa dye. Two thousand cells were analyzed per slide (1000x), and nuclear... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenotoxicity; Nuclear abnormalities; Micronuclei; Centropomus parallelus. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100023 |
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| CRISPIM,BRUNO A.; SPÓSITO,JULIANA C.V.; MUSSURY,ROSILDA M.; SENO,LEONARDO O.; GRISOLIA,ALEXÉIA B.. |
| Anatomical alterations in leaves and DNA damage in cells caused by the accumulation of atmospheric pollutants can be measured by epidermal leaf analyses and Tradescantia micronuclei assay with early pollen tetrad cells. The present study examined the feasibility of using somatic and germ cells of Tradescantia pallida for biomonitoring purposes in the city of Dourados, state of Mato Grosso do Sul (MS), Brazil. Stomatal, micronucleus and epidermal leaf analyses were performed, using standard methodologies, on plants growing at three locations during six different time periods. Tradescantia micronuclei data were analyzed using SAS 9.2 software package and stomatal data were analyzed using SANEST software. Analyses of stomatal characteristics and micronuclei... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biomonitoring; Leaf anatomy; Air pollution; Micronuclei. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401899 |
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| Wang,QL; Zhang,LT; Zou,JH; Liu,DH; Yue,JY. |
| The effects of various cadmium (Cd) concentrations (10, 50 and 100 μM) on root growth, cell division and micronuclei formation in root tip cells of Allium cepa var. agrogarum L. were investigated to better understand the processes of Cd-induced apoptosis. The results indicated that 10 μM of Cd had an obviously passive influence on root growth during the 24 h treatment, and that the influence was even more serious with increasing Cd concentrations and duration of treatments. The mitotic index decreased with increasing Cd concentration and duration of treatments. Cadmium induced c-mitosis, chromosome bridges, chromosome stickiness and micronuclei. The frequency of cells with these disorders varied with prolonging the duration of treatments... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium cepa var. agrogarum L; Cadmium (Cd); Chromosome disorder; Micronuclei. |
| Ano: 2014 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572014000200009 |
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| OLIVEIRA,Angellica Fernandes de; CASTOLDI,Lindsey; VIEIRA JUNIOR,Gerardo Magela; MONÇÃO FILHO,Evaldo dos Santos; CHAVES,Mariana Helena; RODRIGUES,Domingos de Jesus; SUGUI,Marina Mariko. |
| ABSTRACT The skin secretion from toads of the Bufonidae family has great potential in the search for new active compounds to be used as drug candidates in treating some diseases, among them cancer. In this context, this study aimed to evaluate the cytotoxic and antimutagenic activity of the parotoid gland secretion extracts of Rhinella marina and Rhaebo guttatus, as well as biochemically analyze transaminases and serum creatinine for liver and renal damage, respectively. Cytotoxicity was performed by the colorimetric method based on MTT (3- [4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide) with different concentrations of the extracts in Walker or splenic tumor cell cultures from rats and mice. The micronucleus test was performed with male... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotoxicity; Bioprospecting; Micronuclei; Chemoprevention. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000200145 |
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| Guterres,Zaira da Rosa; Mantovani,Mário Sérgio; Eira,Augusto Ferreira da; Ribeiro,Lúcia Regina; Jordão,Berenice Quinzani. |
| Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 -> 6)beta-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agaricus blazei; Micronuclei; Comet assay; V79 cells; Mushroom extracts. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300022 |
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| Poli,M.N; López Miranda,L.A; Fernández Iriarte,P.J; Zanier,G.J; Iúdica,C.E. |
| Previous research has shown connections between the exposure to different agents and mutations in germinal and somatic cells. One of such agents, nitrate (NO3), can be potentially genotoxic for individuals who drink high nitrate well water, and may cause a subsequent impact on their health and on the whole ecosystem. The aim of this research was to conduct genotoxicity assays in somatic cells from people living in the northern area of Mar del Plata city, Argentina. The purpose of these experiments was, in turn, to establish the possible relationships between mutagenic effects and high nitrate water consumption. To this end, different diagnostic tests were carried out to detect potential genetic and/or chromosomal alterations. A non-exposed population equal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mutagenic agents; Nitrate; Chromosomal aberrations; Genotoxicity; Micronuclei. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332012000100001 |
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| Alves dos Santos,Raquel; Cabral,Teresinha Rosa; Cabral,Isabel Rosa; Greggi Antunes,Lusânia Maria; Pontes Andrade,Cristiane; Cerqueira dos Santos Cardoso,Plínio; de Oliveira Bahia,Marcelo; Pessoa,Claudia; Martins do Nascimento,José Luis; Rodríguez Burbano,Rommel; Satie Takahashi,Catarina. |
| Physalis angulata L (Solanaceae) is a medicinal plant from North of Brazil, whose different extracts and infusions are commonly used in the popular medicine for the treatment of malaria, asthma, hepatitis, dermatitis and rheumatism. However, the genotoxic effects of P. angulata on human cells is not well known. The main purpose of the present study was to evaluate the in vitro genotoxic effects of aqueous extract of P. angulata using the comet assay and the micronucleus assay in human lymphocytes provided from 6 healthy donors. Treatments with P. angulata extracts were performed in vitro in order to access the extent of DNA damage. The comet assay has shown that treatments with P. angulata at 0.5, 1.0, 2.0, 3.0 and 6.0 μg/mL in culture medium were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: P. angulata; Micronuclei; Comet assay. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200005 |
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| Haveric,Sanin; Hadzic,Maida; Haveric,Anja; Mijanovic,Mirjana; Hadziselimovic,Rifat; Galic,Borivoj. |
| ABSTRACT Genotoxic effects of inorganic molecule dipotassium-trioxohydroxytetrafluorotriborate, K2(B3O3F4OH), a promising new therapeutic for the epidermal changes treatment, have been evaluated. In vitro analysis included evaluation of genotoxic and cytotoxic potential of K2(B3O3F4OH) in concentrations of 0.01, 0.02, 0.05 and 0.06 mg/mL applying cytokinesis-block micronucleus cytome assay in human lymphocyte culture. With the increase of concentration the frequency of micronuclei elevated but the differences were not significant. Also, there were no significant differences among the frequencies of nuclear buds and nucleoplasmic bridges between controls and treated cultures. Nuclear division index and nuclear division cytotoxycity index values did not... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotoxicity; Micronuclei; Halogenated boroxine; Human lymphocyte culture; BALB/c mice. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100348 |
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| Grisolia,Cesar Koppe; Oliveira,Adriano Barros Barata de; Bonfim,Helder; Klautau-Guimarães,Maria de Nazaré. |
| An evaluation of the genotoxicity of domestic sewage was carried out in a municipal wastewater treatment plant. The study was carried out using Allium cepa root tip cells through cytological parameters such as aberrant cells in anaphase-telophase and the mitotic index, and the fish species Oreochromis niloticus and Tilapia rendalli, through the index of micronuclei in peripheral erythrocytes. In the Allium test, each of the four stages of the wasterwater treatment routine was analyzed, i.e., crude sewage, primary effluent, secondary effluent, and tertiary effluent. O. niloticus and T. rendalli specimens were maintained in an aquarium continuously receiving the final effluent, in an open system, before being discharged into Lake Paranoá. For micronuclei... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium cepa; Biomonitoring; Domestic sewage; Fish; Micronuclei; Mutagenicity. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200026 |
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| Santos Filho,Ronaldo dos; Vicari,Taynah; Santos,Samuel A.; Felisbino,Karoline; Mattoso,Ney; Sant’Anna-Santos,Bruno Francisco; Cestari,Marta Margarete; Leme,Daniela Morais. |
| Abstract Titanium dioxide nanoparticles (TiO2NPs) are widely used and may impact the environment. Thus, this study used a high concentration of TiO2NP (1000 mg/L) to verify the defense mechanisms triggered by a plant system – an indicator of toxicity. Furthermore, this study aimed at completely characterizing TiO2NP suspensions to elucidate their toxic behavior. TiO2NPs were taken up by meristematic cells of Allium cepa, leading to slight inhibition of seed germination and root growth. However, severe cellular and DNA damages were observed in a concentration-dependent manner (10, 100, and 1000 mg/L). For this reason, we used the highest tested concentration (1000 mg/L) to verify if the plant cells developed defense mechanisms against the TiO2NPs and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosomal aberrations; Micronuclei; Nucleolar alterations; Cellular alterations; Nanoparticle phase-change. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300425 |
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| Seriani, Robson; Instituto de Pesca; Ranzani-Paiva, Maria José Tavares; Instituto de Pesca; Silva-Souza, Ângela Teresa; UEL; Napoleão, Silvia Roseli; Instituto de Pesca. |
| This study aimed to determine the variables leukocytary, erythrocytary, frequency of micronuclei and nuclear abnormalities in peripheral erythrocytes of Prochilodus argenteus, Pimelodus maculates and Myleus micans from São Francisco river basin, Minas Gerais State, during the summer and winter. Thrombocytes, hematocrit and leukocyte of P.argenteus series, was influenced by climatic conditions. In P. maculatus, not were significant differences for all leukocytes and thrombocytes. In M. micans, values are unprecedented in the literature, with MCV and absolute number of leukocytes found superior to other species of the family Characidae. The frequency of micronuclei and nuclear abnormalities in erythrocytes, the three species not showed seasonal differences... |
| Tipo: Estudo ecofisiológico |
Palavras-chave: Hematological; Fish; Micronuclei; Nuclear abnormalities; São Francisco Recursos Pesqueiros Hematological; Fish; Micronuclei; Nuclear abnormalities; São Francisco. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7117 |
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| Registros recuperados: 28 | |
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